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Clinical Chemistry 16: 998-1007, 1970;
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Clinical Chemistry, Vol 16, 998-1007, Copyright © 1970 by the American Association for Clinical Chemistry

Measurement of Total Calcium in Serum by Atomic Absorption Spectrophotometry, with Use of a Strontium Internal Reference

John Pybus 1, Fredric J. Feldman 1, and George N. Bowers Jr. 1

1 Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Conn. 06115.

A method for total calcium in serum was optimized, a dual double-beam atomic absorption spectrophotometer being used, with strontium as an internal reference. The effect of HCl and lanthanum concentrations on the signals of both calcium and strontium was determined. The final analysis was unaffected by known sources of interference in serum at a 50-fold dilution of serum in 50 mmolar HCl containing 10 mmol LaCl3 and 0.12 mmol of SrCl2 per liter. Within-run precision for 30 replicate analyses performed during 30 min gave a relative standard deviation (RSD) of 0.36% for the internal strontium reference mode vs. 0.72% RSD for the single-channel mode. Nine consecutive multiconcentration control pools were measured repeatedly for two years; the median RSD was 2.3%. With meticulous technique, long-term variability for total calcium in serum approached 1%. Accuracy is believed to be limited only by the precision, since systematic bias was not detected from recovery studies and comparisons with results of ashing procedures or oxalate precipitation techniques.


Key Words: optimal conditions • factors affecting the analysis • comparison with published AAS procedures for Ca • sources of interference and contamination • normal range • microchemical application

Submitted on August 19, 1970
Accepted on October 1, 1970




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