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Clinical Chemistry 16: 111-117, 1970;
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Clinical Chemistry, Vol 16, 111-117, Copyright © 1970 by the American Association for Clinical Chemistry

Quantitation of Plasma Testosterone by Improved Competitive Protein-Binding Technique

J. A. Demetriou 1 and F. G. Austin 1

1 Research Dept., Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

A method is described for quantitation of testosterone in plasma from females and males. The principal operations of extraction, chromatographic fractionation, and competitive protein-binding assay can be completed for eight duplicate samples in a single day. Experimental data used in the development of the test and the rationale for the specific operations and conditions are presented. The specificity of the method was established by comparing plasma testosterone concentrations determined by the double isotope derivative technique. Concentrations are reported for normal female and male subjects, and for patients with hirsutism, adrenal hyperplasia, Klinefelter’s syndrome, and other endocrine conditions.

Submitted on October 27, 1969
Accepted on November 25, 1969




The following articles in journals at HighWire Press have cited this article:


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R. M. Rose, T. P. Gordon, and I. S. Bernstein
Plasma Testosterone Levels in the Male Rhesus: Influences of Sexual and Social Stimuli
Science, November 10, 1972; 178(4061): 643 - 645.
[Abstract] [PDF]


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Copyright © 1970 by the American Association for Clinical Chemistry.