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Clinical Chemistry 16: 135-138, 1970;
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Clinical Chemistry, Vol 16, 135-138, Copyright © 1970 by the American Association for Clinical Chemistry

Concurrent Assay of Phenobarbital and Diphenylhydantoin in Plasma by Vapor-Phase Chromatography

J. C. Van Meter 1, H. S. Buckmaster 1, and L. L. Shelley 1

1 Mosier Research Laboratory, New Castle State Hospital, New Castle, Ind. 47362.

The weakly acidic phenobarbital and diphenylhydantoin, separated from interfering substances by simple solvent partition, are estimated concurrently by vapor-phase chromatography. Solid sample injection and internal standard (primidone) are used. The entire procedure can be completed within 2 h after venipuncture. Both drugs could be measured after therapeutic doses have been administered.

Submitted on October 24, 1969
Accepted on November 28, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.