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Clinical Chemistry 16: 139-143, 1970;
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Clinical Chemistry, Vol 16, 139-143, Copyright © 1970 by the American Association for Clinical Chemistry

Measurement of Serum Lithium by Atomic Absorption Spectroscopy

John Pybus 1 and George N. Bowers Jr. 1

1 Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Conn. 06115.

Lithium concentrations in the serum of patients undergoing therapy with lithium salts were measured by atomic absorption spectroscopy. Serum was diluted 10-fold with water. Physiological amounts of sodium and potassium were included in the blank and standard solutions because these cations enhance the lithium signal by 2%. Calcium, magnesium, bicarbonate, sulfate, and phosphate at the concentrations found in serum were without effect. Protein clogging of the triple-slot Boling burner was not observed. The relative standard deviation of within-run variability was 0.6% (n = 20 and x = 0.88 mEq/liter), and of daily precision, tested over a month, it was 2.5% (n = 30, x = 0.91 mEq/liter). Recovery of 1.00 mEq of lithium added per liter of serum ranged from 97 to 103%, averaging 99.8%.

Submitted on December 8, 1969
Accepted on December 15, 1969




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