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Clinical Chemistry 16: 148-149, 1970;
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Clinical Chemistry, Vol 16, 148-149, Copyright © 1970 by the American Association for Clinical Chemistry

Adaptation of the Zak-Epstein Automated Micromethod for Serum Iron to Determine Iron-Binding Capacity and Urinary Iron

Jerry P. Kunesh 1 and Larry L. Small 1

1 Department of Physiology and Pharmacology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50010.

An automated method has been developed for determining serum iron-binding capacity and urinary iron as well as serum iron. After preliminary preparation, the sample is treated with ascorbic acid in hydrochloric acid to release and reduce protein-bound iron, then singly dialyzed into an acetate buffer and reacted with 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline) to form a pink color proportionate to the amount of iron present. The sample passes through a 15-mm flow cuvet and is read at 537.5 nm, with the range expander set at either 2x or 4x.

Submitted on October 30, 1969
Accepted on November 24, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.