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Clinical Chemistry 16: 97-102, 1970;
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Clinical Chemistry, Vol 16, 97-102, Copyright © 1970 by the American Association for Clinical Chemistry

Simplified Procedure for Determination of Estrogen in Pregnancy Urine

George F. Grannis 1 and Richard P. Dickey 1

1 Depts. of Pathology and of Obstetrics and Gynecology, The Ohio State University, Columbus, Ohio 43210.

Determination of total estrogen in pregnancy urine has involved hydrolysis, extraction of estrogen, development of color, and extraction of chromogen prior to absorbance measurements. The necessity for each of these steps was evaluated and a more simple procedure was developed which requires only extraction of estrogen and development of color. The new procedure should be useful for prenatal screening and monitoring of high-risk pregnancies.

Submitted on October 10, 1969
Accepted on November 10, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.