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Clinical Chemistry 16: 158-160, 1970;
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Clinical Chemistry, Vol 16, 158-160, Copyright © 1970 by the American Association for Clinical Chemistry

Three Hydrolysis Methods for 17-Ketosteroid Sulfates Compared by Colorimetric and Gas—Liquid Chromatographic Analyses

C. A. Muehlbaecher 1 and E. K. Smith 1

1 Children’s Orthopedic Hospital and Medical Center, and Department of Pediatrics, University of Washington, Seattle, Wash. 98105.

Three methods for cleavage of 17-ketosteroid sulfates were evaluated for their acceptability in the preparation of urine extracts for gas chromatography. Recovery after hydrolysis with hot hydrochloric acid was unacceptably low, but solvolysis with ethyl acetate—sulfuric acid or tetrahydrofuran—perchloric acid was equally efficient and satisfactory. The results obtained by four techniques were intercompared: radioactivity measurement, Zimmermann- or Allen-chromogen development, and gas chromatography.

Submitted on June 9, 1969
Accepted on July 17, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.