Clinical Chemistry
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Clinical Chemistry 16: 166-170, 1970;
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Clinical Chemistry, Vol 16, 166-170, Copyright © 1970 by the American Association for Clinical Chemistry

Improved Accuracy in Automated Fluorometric Determination of Plasma Triglycerides

Robert P. Noble 1 and Frank M. Campbell 1

1 Sharon Research Institute and the Sharon Hospital, Sharon, Conn. 06069.

Accuracy of the automated fluorometric measurement of triglyceride by the technique of Kessler and Lederer is limited by the instability of the baseline. Concentration of the alcoholic KOH reagent was modified, and the pump tube manifold altered to provide on-stream mixing of the KOH reagent, which overcame this difficulty. A relatively inexpensive fluorometer with the solid-state photomultiplier microphotometer, modified to provide dual-scale sensitivity, was used to increase precision of measurements. Analytical results for 30 serum samples, taken from three pools of frozen serum, show the accuracy of the measurements to be ±8 mg/100 ml and the standard deviation ±9 mg/100 ml for a single determination.

Submitted on March 25, 1969




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