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Clinical Chemistry 16: 171-176, 1970;
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Clinical Chemistry, Vol 16, 171-176, Copyright © 1970 by the American Association for Clinical Chemistry

Apparatus for Collecting Fractions from Density Differential Interfaces and Its Use in Gravimetric Measurement of Total Myelin

Robert M. Hill 1 and Virginia E. Bruzek 1

1 Mercy Institute of Biomedical Research, Inc., 2920 East 16th Ave., Denver, Colo. 80206.

An apparatus is described for the precise preparation of density differential interfaces and for quantitative collection of samples from these interfaces. A method is described in which the apparatus is used to quantitatively extract and measure myelin from nerve tissue. Myelin is separated from a homogenate of nerve tissue by density differential ultracentrifugation. The washed myelin is dried. The rate of drying undergoes a sharp change when the loss of free water gives place to the loss of structural water. These two rates are used to calculate the quantity of normally hydrated myelin present.

Accepted on December 22, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.