Clinical Chemistry
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Clinical Chemistry 16: 180-184, 1970;
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Clinical Chemistry, Vol 16, 180-184, Copyright © 1970 by the American Association for Clinical Chemistry

Determination of Bilirubin in Amniotic Fluid A New, Simple, and Efficient Method

V. R. Mallikarjuneswara 1, C. A. B. Clemetson 1, and J. J. Carr 1

1 Dept. of Obstetrics and Gynecology and the Dept. of Laboratories, The Methodist Hospital of Brooklyn, N.Y. 11215.

A simple, speedy, and efficient method is described for the quantitative determination of unconjugated bilirubin in amniotic fluid. The method makes it possible to use an uncentrifuged sample and to detect as little as 0.00125 mg of bilirubin per 100 ml of amniotic fluid without interference by hemoglobin. The analysis can easily be completed in 30 min. Bilirubin is completely separated from all contaminants, except in samples that are grossly contaminated with meconium. If such interference is absent only one reading, at one wavelength, is necessary; a scanning spectrophotometer is therefore not required.

Submitted on January 7, 1969
Accepted on April 1, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.