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Clinical Chemistry 16: 191-194, 1970;
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Clinical Chemistry, Vol 16, 191-194, Copyright © 1970 by the American Association for Clinical Chemistry

Isolation and Quantification of Serum Uric Acid by Adsorption Chromatography

Peter A. Simkin 1

1 Department of Medicine, Division, of Arthritis, University of Washington, Seattle, Wash. 98105.

Established methods for determining the serum uric acid concentration are colorimetric, enzymatic, or combination of the two. An alternative method, based on the affinity of purines for polyacrylamide resin, has been developed. Uric acid, more strongly adsorbed than other major serum constituents, elutes later (with phosphate buffer, pH 7) from a polyacrylamide column. The separated serum uric acid is measured by continuously monitoring the ultraviolet absorptivity of the column effluent. Uric acid is separated satisfactorily from its metabolites, a feature useful in studies of the metabolism of radiolabeled uric acid.

Submitted on June 13, 1969
Accepted on July 4, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.