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Clinical Chemistry 16: 201-206, 1970;
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Clinical Chemistry, Vol 16, 201-206, Copyright © 1970 by the American Association for Clinical Chemistry

Fractionation of Human Urine by Gel Chromatography

C. A. Burtis 1, Gerald Goldstein 1, and Charles D. Scott 1

1 Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830. Operated for the U.S. Atomic Energy Commission by the Union Carbide Corp.

High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G-10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequently analyzed by high-resolution anion-exchange chromatography. Each of these fractions contained from 13 to 63 ultraviolet-absorbing constituents.

Submitted on January 29, 1969
Accepted on May 31, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.