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Clinical Chemistry, Vol 16, 207-211, Copyright © 1970 by the American Association for Clinical Chemistry
1 Medical Research Laboratory, Veterans Administration Hospital, and the Department of Medicine, Emory
University School of Medicine, Atlanta, Ga. 30329.
An automated fluorometric determination of tyrosine has been adapted to the measurement of pepsinogen in body fluids. Preparation of the hemoglobin substrate and methods of incubation are similar to those previously described. The major technical improvement is the elimination of precipitation and filtration; instead, dialysis has been incorporated into the automated procedure for determination of tyrosine and tyrosine-containing peptides. This method provides a reliable, simple technique for use with large numbers of serum specimens.
Submitted on May 26, 1969
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