Clinical Chemistry
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Clinical Chemistry 16: 226-228, 1970;
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Clinical Chemistry, Vol 16, 226-228, Copyright © 1970 by the American Association for Clinical Chemistry

A Membrane Ultrafiltration Procedure for Determining Diffusible Calcium in Serum

Genevieve Farese 1, Milton Mager 1, and William F. Blatt 1

1 Biochemistry and Pharmacology Laboratory, U.S. Army Research Institute of Environmental Medicine, Natick, Mass. 01760, and Scientific Systems Division, Amicon Corporation, Lexington, Mass. 02173.

A rapid, simple procedure is described for separating diffusible from proteinbound serum calcium by centrifugation through high-flux ultrafiltration membranes. The mean normal value for ultrafilterable calcium was 57.1% (range 52 to 61%) of the total serum calcium concentration.

Submitted on October 26, 1968
Accepted on April 19, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.