Clinical Chemistry
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Clinical Chemistry 16: 247-250, 1970;
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Clinical Chemistry, Vol 16, 247-250, Copyright © 1970 by the American Association for Clinical Chemistry

An Improved Reagent System for the Measurement of Serum Uric Acid

D. H. Jung 1 and A. C. Parekh 1

1 Clinical Laboratory, Indiana University Medical Center, 1100 West Michigan St., Indianapolis, Ind. 46202.

The reagent is modified as follows: Serum chromogens are destroyed by trisodium phosphate. The use of phosphotungstic acid eliminates the tungstate and sulfate anions, a frequent cause of turbidity. Triethanolamine, added to the carbonate—urea mixture, provides unusual stability of the color through its buffering action. Only 0.2 ml of serum is required. The method is highly reproducible and accurate; analytical results agree closely with those obtained by the uricase or cyanide—urea method.

Submitted on December 3, 1969
Accepted on January 15, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.