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Clinical Chemistry 16: 269-273, 1970;
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Clinical Chemistry, Vol 16, 269-273, Copyright © 1970 by the American Association for Clinical Chemistry

Use of Ion-Exchange Resin in Preparing Erythrocytes for Magnesium Determinations

B. J. Hunt 1 and J. F. Manery 1

1 Department of Biochemistry, University of Toronto, Toronto 5, Canada.

A rapid, accurate method is described for determining magnesium in red blood cells. Dowex 50 resin was used to separate magnesium from calcium and other cellular constituents. Magnesium in the eluate was titrated with EDTA, with Eriochrome Black T as indicator; the end point was determined spectrophotometrically. Magnesium in the erythrocytes of 17 healthy adults averaged 4.57 ± 0.16 (SD) mEq/liter of packed cells, with a range of 4.32 to 4.88, narrower than reported before in the literature.

Submitted on March 15, 1969
Accepted on May 15, 1969




The following articles in journals at HighWire Press have cited this article:


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R. E. Weber, W. Voelter, A. Fago, H. Echner, E. Campanella, and P. S. Low
Modulation of red cell glycolysis: interactions between vertebrate hemoglobins and cytoplasmic domains of band 3 red cell membrane proteins
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R454 - R464.
[Abstract] [Full Text] [PDF]


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Copyright © 1970 by the American Association for Clinical Chemistry.