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Clinical Chemistry 16: 274-276, 1970;
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Clinical Chemistry, Vol 16, 274-276, Copyright © 1970 by the American Association for Clinical Chemistry

Oxidation of Urinary 17-Oxogenic Steroids with Sodium Metaperiodate

Maxwell H. Abernethy 1 and Mary G. Metcalf 1

1 Medical Unit, The Princess Margaret Hospital, Christchurch, New Zealand.

Oxidation of urinary pregnane-17,20-diols (17-oxogenic steroids) by sodium metaperiodate is complete within 10 min at 55°C if the concentration of sodium metaperiodate at the end of the reaction exceeds 1.5 x 10-3M. A safe excess of sodium metaperiodate is present if assay samples, after incubation for 10 min at 55°C in 3 x 10-3M ethylene glycol, give a positive test with starch-iodide paper.

Submitted on May 21, 1969
Accepted on May 30, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.