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Clinical Chemistry 16: 277-279, 1970;
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Clinical Chemistry, Vol 16, 277-279, Copyright © 1970 by the American Association for Clinical Chemistry

In Vitro Formation of Ammonia in Blood of Dog and Man

Bo Prytz 1, Carlo E. Grossi 1, and Louis M. Rousselot 1

1 Department of Surgery of St. Vincent’s Hospital and Medical Center of New York and The New York University School of Medicine, 153 W. 11th St., New York, N. Y. 10011.

A significant (p <0.001) difference was found in the rate of ammonia formation and the amount formed in heparinized dog and human blood at 4°C. The initial ammonia concentration and the increase in ammonia formation with time were correlated for human blood. This in vitro ammonia formation was not prevented by certain enzyme inhibitors, but it continued after cells and plasma were exhaustively dialyzed against water or saline, suggesting that it originates from enzyme action.

Submitted on December 5, 1968
Accepted on May 7, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.