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Clinical Chemistry 16: 280-281, 1970;
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Clinical Chemistry, Vol 16, 280-281, Copyright © 1970 by the American Association for Clinical Chemistry

Protein-Free Filtrates Obtained by Membrane Ultrafiltration

Application to the Determination of Serum Constituents

Genevieve Farese 1 and Milton Mager 1

1 Biochemistry and Pharmacology Laboratory, U.S. Army Research Institute of Environmental Medicine, Natick, Mass. 01760.

Serum was ultrafiltered by centrifugation through size-selective membranes, and the ultrafiltrates were analyzed directly for glucose, urea nitrogen, creatinine, and uric acid. The concentrations obtained were equivalent to their concentration in conventional protein-free filtrates. This procedure eliminates the need for specific precipitating methods required in various analyses, and yields undiluted samples that are free of foreign ions.

Submitted on December 24, 1968
Accepted on March 19, 1969






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