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Clinical Chemistry 16: 282-284, 1970;
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Clinical Chemistry, Vol 16, 282-284, Copyright © 1970 by the American Association for Clinical Chemistry

Automated Determination of Glucose in Serum or Plasma by a Direct o-Toluidine Procedure

Christopher S. Frings 1, Charles R. Ratliff 1, and Ralph T. Dunn 1

1 Medical Laboratory Associates, 1025 S. 18th St., Birmingham, Ala. 35205 (C.S.F. and R.T.D.), and the Department of Clinical Pathology, Scott and White Clinic, Temple, Tex. 76501 (C.R.R.).

An automated determination of glucose with o-toluidine is described. Microquantities (0.04 ml) of plasma or serum are added directly to one reagent, without dialysis, to produce a green color that follows Beer’s law over a wide range of concentrations. This method compares favorably with a manual o-toluidine procedure.

Submitted on April 7, 1969
Accepted on October 3, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.