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Clinical Chemistry 16: 285-290, 1970;
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Clinical Chemistry, Vol 16, 285-290, Copyright © 1970 by the American Association for Clinical Chemistry

An Automated Micromethod for Determination of Serum Glucose, with an Improved o-Toluidine Reagent

Wells R. Moorehead 1 and Edward A. Sasse 1

1 Department of Clinical Pathology, University of Alabama Medical Center, Birmingham, Ala. 35233.

An automated method has been developed in which a new o-toluidine reagent is used for the microanalysis of glucose. The reagent is 1.3 times as sensitive as the o-toluidine reagent commonly used in manual procedures. The reagent does not significantly alter the life expectancy of manifold tubing made of polyvinyl chloride. The automation includes a dialysis step and possesses the desirable features of the manual methods, including the greater specificity as compared with methods based on reducing properties. Results of this automated method obey Beer’s law, are very reproducible (the coefficient of variation is approximately 1.5%), and are not significantly different from those obtained manually. The method requires 75 µl of sample.

Submitted on December 29, 1969
Accepted on January 12, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.