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Clinical Chemistry 16: 291-293, 1970;
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Clinical Chemistry, Vol 16, 291-293, Copyright © 1970 by the American Association for Clinical Chemistry

Free-Electrophoretic Behavior of Urea-Insulin Reaction Products

Fritz Bischoff 1 and Abolfath Khan Bakhtiar 1

1 Chemical Laboratory, Santa Barbara Cottage Hospital Research Institute, Santa Barbara, Calif. 93105.

Five reaction products, previously isolated from an aqueous mixture of insulin and concentrated urea that had been subjected to various conditions of time and temperature, were examined by moving-boundary electrophoresis. Insulin OU and insulin IU separated into major and minor peaks with mobilities identical to those observed by Timasheff et al. (and confirmed by us) for intact crystalline insulin. Insulin IIU showed a major peak (80%) with a mobility of -7.6 x 10-5 cm/s/V/cm. Insulin IIIU showed a major peak with a mobility of -9.2 x 10-5 cm/s/V/cm. On the basis of change in electron charge and the fact that guanidine does not produce the effects of urea, it is postulated that the delayed-acting urea insulin (IIU) is formed by native insulin reacting with the isocyanate ion in urea solution.

Submitted on July 18, 1969
Accepted on August 29, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.