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Clinical Chemistry 16: 294-299, 1970;
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Clinical Chemistry, Vol 16, 294-299, Copyright © 1970 by the American Association for Clinical Chemistry

An Automated Method for Measuring Creatine Phosphokinase Activity in Serum

Ronald K. Wright 1 and Roy L. Alexander Jr. 1

1 Department of Pathology, St. Louis University Hospitals, 1325 S. Grand Blvd., St. Louis, Mo. 63104.

We describe a procedure for automating the determination of creatine phosphokinase (CPK) activity in serum by use of the AutoAnalyzer. Enzyme activity is determined by measuring the creatine phosphate formed from the CPK-catalyzed reaction of creatine with adenosine triphosphate (ATP). The sensitivity of the automated procedure was comparable to that of the manual method. By use of the most favorable concentrations of creatine, ATP, and magnesium ion in the substrate, a linear relationship was obtained between enzyme concentration and enzyme activities up to 600 mU, representing a sixfold improvement over that obtained by the manual method. The degree of correlation between results obtained by the manual and automated procedures is shown.

Submitted on October 21, 1968
Accepted on May 10, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.