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Clinical Chemistry 16: 300-304, 1970;
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Clinical Chemistry, Vol 16, 300-304, Copyright © 1970 by the American Association for Clinical Chemistry

A New Saccharogenic Micromethod for Measurement of Amylase Activity in Biological Fluids

Klaus Lorentz 1 and Detlef Oltmanns 1

1 I. Medizinische Klinik der Medizinischen Akademie, Lübeck, Germany.

To determine serum amylase activity we have quantitatively measured the glucose and maltose hydrolyzed from soluble starch by colorimetrically measuring the reduction of colorless triphenyltetrazolium chloride to a red formazan, which is dissolved in methanol. The method is suitable for use with microsamples of all biological fluids, and is specific for the final products of starch digestion. Values found for sera from 55 apparently healthy blood donors ranged from 0.15 to 1.55 (mean, 0.83; standard deviation, ±0.4) mg of glucose per ml per h, corresponding to 7.5 to 78 Somogyi units.

Submitted on December 13, 1968
Accepted on June 26, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.