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Clinical Chemistry 16: 322-330, 1970;
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Clinical Chemistry, Vol 16, 322-330, Copyright © 1970 by the American Association for Clinical Chemistry

A Fluorometric Determination of Trypsin-Like Amidase Activity and Activity of Trypsin Inhibitors in Serum

Tetsuo Uete 1, Mineko Asahara 1, and Hiroko Tsuchikura 1

1 Kitano Hospital, Tazuke Kofukai Medical Research Institute, Kita-ku, Osaka City, Japan.

The activity of crystalline trypsin and the trypsin-like amidase activity and the activity of trypsin inhibitors in serum were measured fluorometically, with agr-benzoyl-L-arginine-beta-naphthylamide (BANA) as substrate. The method is simpler and more sensitive than the colorimetric method based on the Bratton-Marshall reaction. Serum of pancreatectomized dogs hydrolyzed BANA at a significant rate, which suggests that serum amidases other than trypsin are active in this determination.

Submitted on April 12, 1969
Accepted on June 26, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.