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Clinical Chemistry 16: 331-334, 1970;
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Clinical Chemistry, Vol 16, 331-334, Copyright © 1970 by the American Association for Clinical Chemistry

Conventional and Preparative Electrophoretic Separation of Some Urinary Porphyrins and Porphyrin Precursors

J. Fischl 1, F. Eichhorn 1, A. Ruttenberg 1, and Ch. Major 1

1 Department of Biochemistry, Rappaport Laboratories, Beilinson Hospital, Petah Tikva, Israel.

Urinary porphyrins are concentrated by adsorption on talc, from which they are eluted with ammonium hydroxide. The eluate is electrophoresed on a membrane filter medium in an alkaline buffer (pH 10.5) for 10 to 20 min. Porphobilinogen and dgr-aminolevulinic acid in urine are separated within 90 min by "small-volume" electrophoresis in a pH 2.2 buffer.

Submitted on December 3, 1969
Accepted on January 28, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.