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Clinical Chemistry 16: 339-346, 1970;
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Clinical Chemistry, Vol 16, 339-346, Copyright © 1970 by the American Association for Clinical Chemistry

A Rapid Extraction and Quantification of Total Lipids and Lipid Fractions in Blood and Feces

J. S. Amenta 1

1 Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pa. 15213.

An analytical system for plasma lipids suitable for use in the large and small clinical laboratory is presented. Lipids are extracted into a chloroform— methanol solvent and concentrated into a chloroform phase by a wash with an aqueous CaCl2 solution. This extract is used to estimate total lipids and (after separation by thin-layer chromatography) the major lipid fractions. A single acid dichromate reagent is used for all quantification steps. Normal ranges for the proposed method are presented. The method with minor modifications is also used for quantification of fecal lipids. Lipids in other body fluids can be conveniently analyzed with this flexible method.

Submitted on July 11, 1969
Accepted on October 15, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.