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Clinical Chemistry 16: 466-471, 1970;
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Clinical Chemistry, Vol 16, 466-471, Copyright © 1970 by the American Association for Clinical Chemistry

Disappearance of Glutathione from Serum or Hemoglobin Solutions

Martin D. Sass 1

1 Medical Research Laboratory, Veterans Administration Hospital, 800 Poly Pl., Brooklyn, N.Y. 11209.

Glutathione disappears as a soluble, nonprotein component after it has been added to serum, either in the reduced (GSH) or oxidized (GSSG) form. When GSH is added to serum it is rapidly oxidized to GSSG by some dialyzable component of serum. Glutathione is lost because of interaction of serum proteins with the GSSG formed in this manner or added directly. A similarly rapid loss of glutathione occurs in the presence of hemoglobin only if acetylphenylhydrazine is also present. This loss of glutathione in the presence of a redox drug is the result of a direct interaction between GSH and hemoglobin, in contrast to the reaction occurring in serum. The implications of these findings for hematologic disease are discussed.


Key Words: acetylphenylhydrazine • hematologic disease • nitroprusside method for GSH • enzymatic method for GSSG • dialysis • loss from urine, other biological fluids

Submitted on February 25, 1970
Accepted on March 16, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.