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Clinical Chemistry 16: 495-499, 1970;
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Clinical Chemistry, Vol 16, 495-499, Copyright © 1970 by the American Association for Clinical Chemistry

Application of Fe+2-5-PyridylBenzodiazepin-2-Ones to the Automated Determinations of Serum Iron and Iron-Binding Capacity

Bernard Klein 1, Norman Kleinman 1, and Ronald L. Searcy 1

1 Department of Diagnostic Research, HoffmannLa Roche Inc., Nutley, N.J. 07110.

An automated procedure has been developed for determining serum iron content and iron-binding capacity with a new iron-chelating ligand, a 5-pyridyl-benzodiazepin-2-one derivative. A simplified manifold and flow system, together with improved reagent combinations, offer distinct advantages over existing automated procedures. The results obtained by automated analysis for serum iron and iron-binding capacity agree excellently with those obtained by the corresponding manual procedure.


Key Words: chelation of iron • AutoAnalyzer

Submitted on October 3, 1969
Accepted on April 6, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.