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Clinical Chemistry 16: 587-593, 1970;
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Clinical Chemistry, Vol 16, 587-593, Copyright © 1970 by the American Association for Clinical Chemistry

Emergency Gas—Liquid Chromatographic Determination of Barbiturates and Glutethimide in Serum

H. E. Sine 1, M. J. McKenna 1, T. A. Rejent 1, and M. H. Murray 1

1 Clinical Laboratory, The Genesee Hospital, 224 Alexander St., Rochester, N.Y. 14607 (H.E.S., M.J.McK., and M.H.M.) and the Erie County Toxicology Laboratory, Buffalo, N.Y. 14215 (T.A.R.).

A method is presented for the rapid gas chromatographic determination of barbiturates and glutethimide in serum, suitable for use in clinical emergencies. The sample is prepared for gas chromatography by acidification and a single extraction with chloroform. From 70 to 100% of the various barbiturates and glutethimide are analytically recovered, with a coefficient of variation of 8 to 10% for the method. During a 10-month clinical evaluation (87 requested determinations), 34 analyses (39% of the samples submitted) were positive for barbiturates, glutethimide, or both.

Submitted on January 2, 1970
Accepted on May 1, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.