Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 16: 677-680, 1970;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hulett, L D.
Right arrow Articles by Carison, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hulett, L D.
Right arrow Articles by Carison, T. A.

Clinical Chemistry, Vol 16, 677-680, Copyright © 1970 by the American Association for Clinical Chemistry

Analysis of Compounds of Biological Interest by Electron Spectroscopy

L D. Hulett 1 and T. A. Carison 1

1 Oak Ridge National Laboratory, Oak Ridge, Tenn. 37850. (Operated for the U. S. AEC by Union Carbide Corp.)

Electron spectroscopy involves measuring the energies of electrons ejected when matter is irradiated with photons. One of the more powerful applications of electron spectroscopy is the determination of the binding energies of the inner shells of atoms with soft X-rays. The binding energies of the inner shells are characteristic of the element; thus, a means of qualitative analysis is provided. Quantitative analysis can be done also. In addition, use of high-resolution spectroscopy enables one to determine the oxidation state of each atom, because the binding energies will shift with chemical environment. For example, the K shell binding energy in nitrogen is about 10 V lower for an amine group than for a nitro group. This analysis can be used for all elements with atomic numbers higher than lithium. The advantages of electron spectroscopy for biological research, both for fundamental and in chemical applications, are discussed in general. Recent studies on t-RNA and its bases are given as specific examples of the applicability of the method.


Key Words: t-RNA • nucleic acid analysis • film thickness • zwitterions • electron binding—energy shifts • photoelectron spectrometer






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1970 by the American Association for Clinical Chemistry.