Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 16: 722-725, 1970;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Butts, W. C.
Right arrow Articles by Jolley, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Butts, W. C.
Right arrow Articles by Jolley, R. L.

Clinical Chemistry, Vol 16, 722-725, Copyright © 1970 by the American Association for Clinical Chemistry

Gas-Chromatographic Identification of Urinary Carbohydrates Isolated by Anion-Exchange Chromatography

W. C. Butts 1 and R. L. Jolley 1

1 Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830. (Operated for the U.S. Atomic Energy Commission by Union Carbide Corp.)

More than 40 chromatographic peaks representing carbohydrates have been separated from human urine by a carbohydrate analyzer that makes use of anion-exchange chromatography. Ten of these separated carbohydrates have been identified by gas chromatography of their trimethylsilyl derivatives, which were formed by reaction with N-trimethylsilylimidazole. The borate buffer from the anion-exchange separation was removed from the samples before the derivatives were made. Gas-chromatographic retention data have been determined for 37 carbohydrates, including pentoses, hexoses, hexosamines, disaccharides, and trisaccharides, and are tabulated as methylene unit (MU) values. These various classes of carbohydrates elute within distinct MU regions, generally in the order of increasing molecular weight. More than one peak is obtained for those sugars existing in more than one anomeric form.


Key Words: trimethylsilyl derivatives • "methylene unit" values • molecular weight—elution relationships • N-trimethylsilylimidazole






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1970 by the American Association for Clinical Chemistry.