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Clinical Chemistry 16: 740-742, 1970;
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Clinical Chemistry, Vol 16, 740-742, Copyright © 1970 by the American Association for Clinical Chemistry

Serum Content of Macroglobulins (19S Fraction) and Its Variation with Age when Determined by Thin-Layer Gel-Filtration

Lleni Pach de Goldman 1, Liliana Ballivian 1, and Ernesto Melgar 1

1 Institute of Biochemistry and Nutrition, Department of Biochemistry and Physiology, University of San Marcos, Lima, Peru.

Serum proteins were fractionated by thin-layer gel-filtration on Sephadex G-200, and the content of macroglobulins (19S fraction) was determined in 137 samples from apparently healthy subjects of different ages. The relationship between amount of 19S fraction relative to total protein was found to vary widely with age. This variation has to be considered when the method is used for clinical purposes.


Key Words: normal values • children and adults • Sephadex G-200 • analytical ultracentrifugation compared • umbilical cord blood • lipoproteins, serum • malignant disease

Submitted on March 17, 1970
Accepted on May 25, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.