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Clinical Chemistry 16: 760-762, 1970;
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Clinical Chemistry, Vol 16, 760-762, Copyright © 1970 by the American Association for Clinical Chemistry

Computer Calculation of Serum Protein Electrophoresis, Based on Peak Height Measurements

Sylvan M. Sax 1 and John J. Moore 1

1 Department of Pathology, The Western Pennsylvania Hospital, 4800 Friendship Ave., Pittsburgh, Pa. 15224.

A computer program has been written for off-line calculation of relative and absolute percentages of the five serum protein fractions usually seen on cellulose acetate electrophoretograms, with use of manually observed peak heights on densitometer scans. With myeloma-like peaks, both peak height and width at half-peak height must be measured. Routine application of the program saves technician time and decreases the number of large random errors.


Key Words: relative and absolute percentages • myeloma proteins • electrophoresis on cellulose acetate

Submitted on May 1, 1970
Accepted on June 3, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.