Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 16: 766-772, 1970;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garry, P. J.
Right arrow Articles by Owen, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garry, P. J.
Right arrow Articles by Owen, G. M.

Clinical Chemistry, Vol 16, 766-772, Copyright © 1970 by the American Association for Clinical Chemistry

Plasma Vitamin A Assay by Fluorometry and Use of a Silicic Acid Column Technique

Philip J. Garry 1, J. Dennis Pollack 1, and George M. Owen 1

1 Departments of Pediatrics and Medical Microbiology, College of Medicine, Ohio State University, Columbus, Ohio 43205; and the Children’s Hospital Research Foundation, Columbus, Ohio.

We investigated a new procedure in which silicic acid column chromatography and fluorometry are used to assay plasma vitamin A. Using tritium-labeled vitamin A acetate and vitamin A alcohol as radioactive standards, we identified two fluorescent components in plasma extracts chromatographed on silicic acid columns. One unidentified fluorescent component was eluted first with hexane or petroleum ether. Vitamin A, the second fluorescent peak, was quantitatively eluted with isopropanol. The isopropanol eluates could be immediately assayed fluorometrically without further treatment. The unidentified compound was sensitive to ultraviolet irradiation, which destroyed it slower than vitamin A is destroyed. Values correlated well for plasma vitamin A concentrations, determined by this procedure and by the Neeld and Pearson modification of the Carr-Price colorimetric method (r = 0.871). By our technique, we found 34 µg of vitamin A per 100 ml of plasma in 778 preschool children.


Key Words: tritium-labeled vitamin A • effects of ultraviolet irradiation • concentrations in plasma of preschool children • phytofluene • tritium-labeled retinol • fluorescent compounds in serum • Carr-Price colorimetric method

Submitted on May 18, 1970
Accepted on May 29, 1970




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1970 by the American Association for Clinical Chemistry.