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Clinical Chemistry 16: 773-775, 1970;
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Clinical Chemistry, Vol 16, 773-775, Copyright © 1970 by the American Association for Clinical Chemistry

Quantitation of Sputum Protein by Use of the Biuret Reaction

Joyce P. Fields 1 and Sanford Chodosh 1

1 Lung Station (Tufts), Boston City Hospital and the Department of Medicine, Tufts University School of Medicine, Boston, Mass. 02118.

A simple, accurate method is described for quantitatively determining the protein content of sputum by use of the biuret reaction. Fifteen mechanically homogenized specimens were examined with the biuret reagent described by Rosenthal and Cundiff, and the results compared with those obtained by the microKjeldahl technique. Over a range of 542 to 1917 mg of protein per 100 ml, the techniques correlated significantly. Difficulties with deproteinization of the sputum before nonprotein nitrogen was determined were overcome by the use of zinc sulfate and pH adjustment. The proposed method is fast and reproducible.


Key Words: microKjeldahl results compared • nonprotein nitrogen content • EDTA stabilizer • variation among patients

Submitted on April 27, 1970
Accepted on June 5, 1970




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[Abstract] [Full Text] [PDF]


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Copyright © 1970 by the American Association for Clinical Chemistry.