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Clinical Chemistry 16: 776-780, 1970;
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Clinical Chemistry, Vol 16, 776-780, Copyright © 1970 by the American Association for Clinical Chemistry

Quantification of Serum Inorganic Phosphorus, Phosphatase, and Urinary Phosphate without Preliminary Treatment

Jesse F. Goodwin 1

1 General Clinical Research Center for Children of the Children’s Hospital of Michigan, and Wayne State University School of Medicine, Detroit, Mich. 48202.

A direct method has been devised for determining serum and urinary inorganic phosphorus and serum phosphatase activity. Inorganic phosphorus forms a phosphomolybdate complex in the presence of borate. The complex is reduced with ascorbic acid. The resulting suspension is solubilized with carbonate and the absorbance measured at 720 nm. Samples containing low to moderate concentrations of bilirubin may be assayed by the method, and it compares favorably with a p-semidine reduction procedure with use of a TCA filtrate. Alkaline phosphatase values obtained by the method in which a beta-glycerophosphate substrate is used compare favorably with a reference method in which p-nitrophenolphosphate is used as a substrate. Citrates, fluorides, nitrites, oxalates, and mannitol do not appreciably affect results obtained by the method.


Key Words: p-nitrophenolphosphate method compared • possible micromethod • Ringbom plot • bilirubin interference • acid and alkaline phosphatase • urinary Pi value for an infant

Submitted on April 20, 1970
Accepted on June 9, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.