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Clinical Chemistry 16: 798-799, 1970;
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Clinical Chemistry, Vol 16, 798-799, Copyright © 1970 by the American Association for Clinical Chemistry

Absence of Measurable Leukocyte Alkaline Phosphatase Activity from Leukocytes of Patients with Chronic Granulocytic Leukemia

Lawrence R. DeChatelet 1, M. Robert Cooper 1, and Charles E. McCall 1

1 Departments of Biochemistry and Medicine, The Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, N.C. 27103.

Leukocyte phosphatase activity was determined over a broad pH range. Using this procedure, we found no alkaline phosphatase activity in leukocytes from three patients with chronic granulocytic leukemia. The low values usually reported for this disease are actually attributable to acid phosphatase activity, which is slight but measurable at alkaline pH.


Key Words: sonication of cells • spectrophotometry • acid phosphatase activity of leukocytes

Submitted on June 25, 1970
Accepted on July 8, 1970




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Copyright © 1970 by the American Association for Clinical Chemistry.