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Clinical Chemistry 17: 37-41, 1971;
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Clinical Chemistry, Vol 17, 37-41, Copyright © 1971 by the American Association for Clinical Chemistry

Isolation of Urinary 17-Ketosteroid Conjugates by Ammonium Sulfate Precipitation

Geraldine S. Pinkus 1 and Jack L Pinkus 1

1 Departments of Pathology (Presbyterian—University Hospital) and of Obstetrics and Gynecology (Magee Womens Hospital), University of Pittsburgh School of Medicine, Pittsburgh, Pa. 15213.

Ammonium sulfate precipitation of urinary 17-ketosteroid conjugates is a rapid, effective method for isolating and purifying them. Negligible quantities of pigment are produced during acid hydrolysis of solutions of the precipitated conjugates. Despite elimination of nonspecific chromogenic material, colorimetric determinations of Zimmerman chromogens with use of the Allen correction of absorbances [J. Clin. Endocrinol 10, 71 (1950)] gave comparable 17-ketosteroid values for solutions of the precipitate and for urines. Recoveries of urinary 17-hydroxysteroid conjugates were variable when the ammonium sulfate procedure was used.


Key Words: colorimetry of Zimmerman chromogens • 17-hydroxysteroid analysis • use of radiolabeled steroids

Submitted on August 31, 1970
Accepted on October 22, 1970






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Copyright © 1971 by the American Association for Clinical Chemistry.