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Clinical Chemistry 17: 51-52, 1971;
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Clinical Chemistry, Vol 17, 51-52, Copyright © 1971 by the American Association for Clinical Chemistry

Diagnosis of a Case of Acute Intermittent Porphyria with the Aid of Thin-Layer Chromatography—Thin-Layer Electrophoresis of Urine

Stanley Samuels 1 and Gaston Veliz 1

1 Department of Neurology, New York University Medical Center, New York, N.Y. 10016.

dgr-Aminolevulinic acid and porphobilinogen were identified in urine from a patient with acute intermittent porphyria on routine amino acid chromatography. The compounds were well and rapidly separated by a two-dimensional combination of high-voltage, thin-layer electrophoresis and chromatography. Both compounds gave a yellowish color with ninhydrin; the porphobilinogen gave a strong red color when Ehrlich’s reagent was then applied. Such a pattern has not been observed in any blood and urinary amino acid chromatograms from over 900 patients with other conditions, who were receiving a variety of drugs. We consider this a rapid, specific diagnostic aid in cases of acute intermittent porphyria.


Key Words: dgr-aminolevulinic acid • porphobilinogen • ninhydrin • Ehrlich’s reagent

Accepted on October 19, 1970






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Copyright © 1971 by the American Association for Clinical Chemistry.