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Clinical Chemistry 17: 1010-1015, 1971;
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Clinical Chemistry, Vol 17, 1010-1015, Copyright © 1971 by the American Association for Clinical Chemistry

Glucose Assay Systems: Evaluation of a Colorimetric Hexokinase Procedure

Walter R. Wright 1, John C. Rainwater 1, and Lawrence D. Tolle 1

1 Department of Pathology, East Tennessee Baptist Hospital, Knoxville, Tenn. 37920.

A colorimetric enzymatic assay system for glucose was evaluated. The system uses hexokinase (ATP:D-hexose 6-phospho-transferase; EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADP oxidoreductase; EC 1.1.1.49) coupled with a PMS-INT reaction (phenazine methosulfate; 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride). The precision, range of linearity, reproducibility of standard curve, and stability of reagents after reconstitution are excellent. The method is specific for glucose in the presence of mannose, fructose, galactose, xylose, and ribose. Uric acid, urea, ascorbic acid, reduced glutathione, or creatinine at abnormally high concentrations do not interfere. Good correlations (with unselected hospital sera) were obtained in parallel assays by ferricyanide (AutoAnalyzer), o-toluidine (manual), and hexokinase (manual) procedures. Results obtained by the neocuproine (SMA-12/30) method in the parallel assays were significantly different from results obtained by the other three procedures.


Key Words: intermethod comparison • "GlucoStrate" • "Glytel"

Submitted on March 30, 1971
Accepted on June 30, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.