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Clinical Chemistry 17: 1038-1041, 1971;
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Clinical Chemistry, Vol 17, 1038-1041, Copyright © 1971 by the American Association for Clinical Chemistry

Improved Method for Measurement of delta-Aminolevulinic Acid Dehydratase Activity of Human Erythrocytes

Helen B. Burch 1 and Abraham L. Siegel 1

1 Trace Metals Laboratory, Veterans Administration Hospital, Birmingham, Ala., and the Department of Clinical Pathology, University of Alabama School of Medicine, Birmingham, Ala. 35233.

We have evaluated and modified a current method for estimating dgr-aminolevulinic acid dehydratase (5-aminolaevulinate hydro-lyase, EC 4.2.1.24) activity in blood, so as to adequately control the pH of the enzyme-substrate solution at the optimum throughout the incubation period. This has improved the sensitivity and reliability of the assay. Also, N-ethylmaleimide has been substituted for a potentially hazardous mercury salt, used to remove sulfhydryl groups before color development with a modified Ehrlich’s reagent.


Key Words: N-ethylmaleimide as —SH binder • phosphate—citrate buffer

Submitted on June 11, 1971
Accepted on July 23, 1971




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