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Clinical Chemistry 17: 1050-1054, 1971;
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Clinical Chemistry, Vol 17, 1050-1054, Copyright © 1971 by the American Association for Clinical Chemistry

Manual Procedure for Direct Microassay of Serum Glucose by Use of o-Toluidine, and Its Adaptation to the SMA 12/60 AutoAnalyzer

W. W. Webster 1, S. F. Stinson 1, and W. H. Wong 1

1 Clinical Laboratory Medical Group, 679 South Westlake Ave., Los Angeles, Calif. 90057; and the Department of Pathology, The Hospital of the Good Samaritan Medical Center, Los Angeles, Calif. 90017.

A direct o-toluidine method for serum glucose is described as an alternative to the usual Cu—neocuproine method used with the Technicon SMA 12/60 AutoAnalyzer. Routine operation of the machine is simplified: the number of reagents and manifold lines is decreased and the dialyzer and pre-heating bath are eliminated. Deterioration of manifold tubing is minimized, while maintaining optimal machine sensitivity by adding borate and water to the reagent. Serum glucose was measured by the automated o-toluidine and Cu—neocuproine methods with similar, highly correlated results. Thus, the "normal limits" are unchanged and the same recorder graph paper may be used. A direct manual micro-method is also described, in which an anhydrous o-toluidine reagent is used. Results of manual and automated methods agree satisfactorily.


Key Words: Cu—neocuproine method • Beckman Glucose Analyzer • thiourea/borate in reagent

Submitted on April 13, 1971
Accepted on August 2, 1971




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Copyright © 1971 by the American Association for Clinical Chemistry.