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Clinical Chemistry 17: 1059-1060, 1971;
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Clinical Chemistry, Vol 17, 1059-1060, Copyright © 1971 by the American Association for Clinical Chemistry

Murayama Test for Hemoglobin S: Simplification in Technique

Bruce M. Nichols 1, Robert M. Nalbandian 1, Raymond L. Henry 1, Paul L. Wolf 1, and Frank R. Camp Jr. 1

1 Department of Pathology, Blodgett Memorial Hospital, Grand Rapids, Mich. 49506 (B.M.N., R.M.N.); the School of Medicine, Wayne State University, Detroit, Mich. 48207 (R.L.H. and R.M.N.); Stanford University Medical Center, Stanford, Calif. 94305 (P.L.W.); and the Blood Transfusion Division, U.S. Army Medical Research Laboratory, Fort Knox, Ky. 40121 (F.R.C.).

An improved technique is reported for the Murayama test, which is a simple and specific test for S hemoglobin [Clin. Chem. 16, 945 (1970)]: moisturized N2 is used optimally and several steps are eliminated in the part of the test in which concentrated hemolysates are prepared by use of a dense sucrose solution. Each test, which took 1 h, can now be done in 20 min, with equal reliability and accuracy.

Submitted on June 7, 1971
Accepted on July 6, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.