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Clinical Chemistry 17: 994-997, 1971;
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Clinical Chemistry, Vol 17, 994-997, Copyright © 1971 by the American Association for Clinical Chemistry

Automated Simultaneous Turbidimetric Determination of Cholesterol in beta- and pre-beta-Lipoproteins

A. Lopez 1, R. Vial 1, L. Gremillion 1, and L. Bell 1

1 Departments of Medicine and Biochemistry, Louisiana State University School of Medicine, New Orleans, La. 70112.

An automated method is described for simultaneously determining cholesterol in serum beta- and pre-beta-lipoprotein fractions. This fast, reproducible, accurate method depends on the ability of beta- and pre-beta-lipoproteins to form insoluble complexes with polyanionic polysaccharides such as heparin. Values obtained by this method and by similar manual methods described in the literature agree well. This method is extremely useful in mass screening for hyperlipidemias; in certain cases it may be more useful than the determination of only cholesterol or triglycerides.


Key Words: AutoAnalyzer • complexes with heparin • hyperlipidemias • mass screening

Submitted on May 10, 1971
Accepted on June 28, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.