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Clinical Chemistry 17: 1081-1082, 1971;
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Clinical Chemistry, Vol 17, 1081-1082, Copyright © 1971 by the American Association for Clinical Chemistry

Rapid Solubility Test for Detection of Hemoglobin S

Jean E. Matusik 1, James B. Powell 1, and David M. Gregory 1

1 Division of Biochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012.

We have devised a rapid screening test for use in detecting sickling hemoglobins. This test depends on the insolubility of sickling hemoglobins in 2.24 molar phosphate buffer. Sickling hemoglobins form a turbid suspension in this test, in which positive and negative results, respectively, appear very much like those for the "Sickledex" test.


Key Words: solubility of Hgb S • electrophoresis • techniques for detecting hemoglobin S compared • "Sickledex" • sickling hemoglobins • tests for sickle cell disease • turbidimetric tests

Submitted on July 5, 1971
Accepted on August 17, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.