Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 17: 1083-1088, 1971;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holub, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holub, W. R.

Clinical Chemistry, Vol 17, 1083-1088, Copyright © 1971 by the American Association for Clinical Chemistry

Routine Gas—Liquid Chromatographic Method for Simultaneous Assay of Major Urinary 17-Ketosteroids, and Pregnanediol and Pregnanetriol

William R. Holub 1

1 Endocrine Function Analysis Division of Central Health Laboratories, Inc., 636 Wantagh Ave., Levittown, N.Y. 11756, and St. Johns University, Jamaica, N.Y. 11432.

Many methods of urinary steroid analysis have been used in evaluating endocrine function. Individual determination of particular metabolites and functional group analysis of steroid groups frequently involves extensive isolation and correction for interferences. By utilizing the separative and quantitative capabilities of gas-liquid chromatography, we have developed a method for the simultaneous analysis of certain clinically significant urinary steroids. Each analytical step has been made optimal, resulting in a simple and rapid method for determining these steroid metabolites in urine. Although it is a good technique for isolating and concentrating steroid conjugates, ammonium sulfate precipitation of the steroid conjugates is frequently unnecessary. Use of sulfatase and glucuronidase provides the most efficient and "clean" hydrolytic procedure.


Key Words: hydrolysis and extraction, results compared by GLC (NH4)2SO4 precipitation of steroids • steroid conjugates

Submitted on April 29, 1971
Accepted on July 24, 1971






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1971 by the American Association for Clinical Chemistry.