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Clinical Chemistry 17: 1114-1122, 1971;
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Clinical Chemistry, Vol 17, 1114-1122, Copyright © 1971 by the American Association for Clinical Chemistry

Serum Glutamic—Oxaloacetic Transaminase: Evaluation of a Coupled-Reaction Enzyme Assay by Means of Kinetic Theory

C. D. Russell 1 and E. Cotlove 1

1 Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Md. 20014.

Rate equations and rate parameters are determined for the coupled-reaction assay for serum glutamic-oxaloacetic transaminase in which malic dehydrogenase is used. These are used to interpret observed curves of absorbance vs. time, to select optimum substrate concentrations, and to estimate nonrandom analytical errors. A simple, systematic, general approach is presented, which can readily be applied to other enzyme assays in which two or more reactions are coupled. It depends for its simplicity on access to an adequate computing facility.


Key Words: computer-assisted selection of optimal conditions for enzymatic reactions • absorbance curves, theoretical and observed • malate dehydrogenase

Submitted on June 21, 1971
Accepted on August 18, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.