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Clinical Chemistry 17: 1150-1153, 1971;
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Clinical Chemistry, Vol 17, 1150-1153, Copyright © 1971 by the American Association for Clinical Chemistry

Simplified Turbidimetric Assay for Lipase Activity

Zakariya K. Shihabi 1 and Charles Bishop 1

1 Biochemistry Laboratory of the Buffalo General Hospital, Buffalo, N. Y. 14203 and the Department of Medicine of the State University of New York at Buffalo, Buffalo, N. Y.

Serum lipase is determined by following turbidity changes during two 1-min intervals after adding serum to an olive oil emulsion containing desoxycholate. The olive oil emulsion is simply prepared and is stable for a month under refrigeration. Our observations confirm the findings of other investigators that increases in serum lipase activity are more accentuated than increases in serum amylase activity during pancreatitis. With the present fast and convenient method, serum lipase appears to be a better test for pancreatitis than is serum amylase.


Key Words: amylase • pancreatitis • normal values

Submitted on July 14, 1971
Accepted on August 19, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.