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Clinical Chemistry 17: 1154-1159, 1971;
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Clinical Chemistry, Vol 17, 1154-1159, Copyright © 1971 by the American Association for Clinical Chemistry

Automated Determination of Uric Acid, with Use of a Uricase—Peroxidase System

Nathan Gochman 1 and Joan M. Schmitz 1

1 Clinical Chemistry Service, Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Md. 20014.

An automated colorimetric procedure is described for the determination of serum and urine uric acid with improved specificity. Hydrogen peroxide, formed when uric acid is oxidized by uricase, oxidatively couples with 3-methyl-2-benzothiazolinone hydrazone and N,N-dimethylaniline, in the presence of peroxidase, to produce a stable, sensitive indamine dye. The method is reproducible, and the results correlate well with those obtained by a manual spectrophotometric uricase procedure. Also, the automated procedure is resistant to many added reducing substances that cause positive interferences in alkaline phosphotungstate methods.


Key Words: AutoAnalyzer • hydrogen-peroxide • normal values • use in uric acid excretion studies

Submitted on August 23, 1971




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